On-chip sort is manufactured by On-chip Biotechnologies Co., Ltd. and marketed in North America by PHC Corporation of North America subsidiary of PHC Corporation in Japan.

Cell Sorter On a Chip

On-chip Sort, the microfluidic chip cell sorter. Damage-, contamination-, and maintenance-free. Safe and high precision cell sorter with the world’s first microfluidic chip technology

Excellent operability and User-friendly maintenance, a cell sorter with brand-new technology. Unlike a conventional cell sorter, all fluids remain on a sterile and disposable microfluidic chip. The microfluidic cell sorter not only resolved the inherent issues of the conventional sorters, but also enabled analysis/sorting of cells suspended in culture medium.

The microfluidic chip­-based flow cytometer has the following advantages:

“Damage­-free” cell sorting
Cells are sorted by On-chip’s unique “flow shift” method in microchannels, avoiding shear stresses, pressure changes, collisions, and strong electrostatic field which might damage or alter the cells.

Simple, sterile cell sorting with no cross-contamination
Because our sterilized microfluidic chips are disposable, cross-contamination between samples does not occur. It is compact enough that it can fit inside a biosafety cabinet, with no generation of aerosols. This is an ideal solution for safe handling of biohazardous materials, where safety is paramount.

Sort cells in culture media
Run the sort with your preferred buffer: culture media, sea water and even oil.

Sample volume as low as 20 μL
Small sample volumes (~20 μL) can be analyzed without almost no dead volume. On-chip Sort is the most suitable instrument for the analysis and sorting of precious samples.

Maintenance-free and easy to use
Maintenance of On-chip Sort is easy. Fluids does not get in contact with the instrument, so no cleanup is required. On-chip Sort is easy to operate and does not require hours of training.

Cell Sorter using microfluidic chip technology

Unlike a conventional cell sorter, all fluids are handled on a sterile and disposable microfluidic chip. Our unique sorting technique enables sorting in damage-free, contamination-free, and maintenance-free manner.

  • Microfluidic technology
  • Principle of On-chip’s novel cell sorting technique
  • Realization of damage-free cell sorting
  • Optical technology
  • Easy maintenance and high flexibility

Supporting a variety of fluorescent colors / High sensitivity

Selectable lasers

Up to three lasers from Blue (primary 488nm), Red (637nm), Violet (405nm) and Green (561nm) lasers can be installed for On-chip Sort. The system has sensitivity of FITC<200 MESF, although using a disposable chip as a flow cell. It has 6 fluorescence detectors and 2 scatters (forward and side).

Number of lasers & detectors

Product name Catalogue No. Lasers No. of detectors
On-chip Sort HS 362S2001 Blue Red Violet 6
On-chip Sort HSG 362S2001G Blue Green Violet 6
On-chip Sort HSGR 362S2001GR Blue Green Red 6
On-chip Sort MS5 252S2001 Blue Red 5
On-chip Sort MS5G 252S2001G Blue Green 5
On-chip Sort MS6 262S2001 Blue Violet 6
On-chip Sort LS5 152S2001 Blue 5

Specifications On-chip Sort

Laser Up to 3 Lasers from 405nm, 488nm, 561nm, 637nm
Measurement parameter Forward scatter (FSC), Side scatter (SSC), 6 FL (up to 10 parameters)
Size sensitivity FSC < 0.5μm, SSC < 1.0 μm
Fluorescence sensibility < 200 MESF FITC
Data resolution 4 decades, 18 bit
Signal type Height, Width, Area
Detection wavelength FL1(445/20 nm), FL2(543/22 nm), FL3(591.5/43 nm)
FL4(676/37 nm), FL5(716/40 nm), FL6(775/46 nm)
Flow-cell chip Disposable micorfluidic chip
Material of chip

Channel dimensions 80 μm × 80 μm and 150 μm × 150 μm
Flow speed
500 mm/sec

Sheath buffer Aqueous solutions, oil etc
Volume of sample 20-1000 μL
Volume of sheath 4-9 mL

Analysis and Sorting
Sorting method “Flow Shift” to generate pulse flow
Purity > 95% (depends on cell concentration)
Yield > 80%
Cell damage No
Cross-contamination No cross-contamination
Sterilized Yes
Pressure 0.3 psi
Detection speed 4,000 events/sec
Sorting speed 1000 target cells collected/sec
Shutdown 10 sec (cleaning of tubing are not necessary)
Generation of aerosol No
Size and Weight
Size (W×H×D) 620mm × 330mm × 390mm
Weight 45 kg
PC and Software
PC and Software Laptop PC
OS Windows, 64 bit
Data format Original, FCS3.0, csv
Supply Power consumption < 240 VA


On-chip Sort: The world’s first Cell Sorter using disposable microfluidic chips for damage-free sorting.
On-chip Biotechnologies has developed cell sorters based on microfluidic chip technology to gently sort even the most delicate cells damage-free. Because all fluids stay contained in the sterile disposable chips, there is no chance of cross contamination and no instrument cleanup.
A conventional cell sorter gives damage to sorted cells due to electric charge and collision. Moreover, It is impossible to perform selection of sheath liquid and sorting of the cell by various culture medium in conventional sorting. In addition, since a flow system is nonexchangeable, cross contamination of a sample arises in conventional sorting. On-chip developed a cell sorting system which adopted microfluidic technology on disposable chips, enabling it to separate precious samples without any damage-free.
We introduce a few applications which show advantages of the equipment.
Find the applications of On-chip Sort.

PHC Corporation of North America

PHC Corporation of North America is a leader in laboratory equipment for the biopharmaceutical, life sciences, academic, healthcare and government markets. Product lines under the new PHCbi brand include the space saving and energy efficient VIP®ECO, TwinGuard®and VIP Series ultra-low temperature freezers, cryogenic and biomedical freezers, pharmacy and high performance refrigerators, cell culture CO2 and multigas incubators, portable autoclaves, cell processing work stations and Drosophila/plant growth chambers. PHC Corporation of North America is a subsidiary of PHC Holdings Corporation, Tokyo, Japan, which is a global healthcare company involved in the three core businesses of Medical Devices, Healthcare IT and Life Sciences. For more product information, please call PHC Corporation of North America at 800-858-8442