Cells are sorted by On-chip’s unique “flow shift” method in microchannels, avoiding shear stresses, pressure changes, collisions, and strong electrostatic field which might damage or alter the cells.

Because our sterilized microfluidic chips are disposable, cross-contamination between samples does not occur. It is compact enough that it can fit inside a biosafety cabinet, with no generation of aerosols. This is an ideal solution for safe handling of biohazardous materials, where safety is paramount.

Run the sort with your preferred buffer: culture media, sea water and even oil.

Small sample volumes (~20 μL) can be analyzed without almost no dead volume. On-chip Sort is the most suitable instrument for the analysis and sorting of precious samples.

Maintenance of On-chip Sort is easy. Fluids does not get in contact with the instrument, so no cleanup is required. On-chip Sort is easy to operate and does not require hours of training.

Unlike a conventional cell sorter, all fluids are handled on a sterile and disposable microfluidic chip. Our unique sorting technique enables sorting in damage-free, contamination-free, and maintenance-free manner.

Selectable lasers

Up to three lasers from Blue (primary 488nm), Red (637nm), Violet (405nm) and Green (561nm) lasers can be installed for On-chip Sort. The system has sensitivity of FITC<200 MESF, although using a disposable chip as a flow cell. It has 6 fluorescence detectors and 2 scatters (forward and side).

Number of lasers & detectors

Product name	Catalogue No.	Lasers	No. of detectors
On-chip Sort HS	362S2001	Blue Red Violet	6
On-chip Sort HSG	362S2001G	Blue Green Violet	6
On-chip Sort HSGR	362S2001GR	Blue Green Red	6
On-chip Sort MS5	252S2001	Blue Red	5
On-chip Sort MS5G	252S2001G	Blue Green	5
On-chip Sort MS6	262S2001	Blue Violet	6
On-chip Sort LS5	152S2001	Blue	5

Specifications On-chip Sort

On-chip Sort: The world’s first Cell Sorter using disposable microfluidic chips for damage-free sorting.
On-chip Biotechnologies has developed cell sorters based on microfluidic chip technology to gently sort even the most delicate cells damage-free. Because all fluids stay contained in the sterile disposable chips, there is no chance of cross contamination and no instrument cleanup.
A conventional cell sorter gives damage to sorted cells due to electric charge and collision. Moreover, It is impossible to perform selection of sheath liquid and　sorting of the cell by various culture medium in conventional sorting. In addition, since a flow system is nonexchangeable, cross contamination of a sample arises in conventional sorting. On-chip developed a cell sorting system which adopted microfluidic technology on disposable chips, enabling it to separate precious samples without any damage-free.
We introduce a few applications which show advantages of the equipment.