PRIMESURFACE®

Ultra Low Attachment 3D cell culture plates

PHC provides superior quality three-dimensional cell culture platforms with a variety of well shapes to enable spheroid culturing of your specific cell type.

PrimeSurface cell culture labware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly of spheroid formation. The plates are pre-coated with unique ultra hydrophilic polymer that enables spontaneous spheroid formation of uniform size and shape. The ULA plates have high optical clarity making them highly suitable for bright field imaging and confocal microscopy. In addition to the widely used 96 well U bottom plate, 96 well plates are also available in V and M bottom, giving scientists a choice to form tighter spheroids that are needed for specific cell types. For high throughput screening (HTS) needs, 384 well plates are available in clear and white.


Key benefits
  • Non-binding surface for cells to facilitate natural spheroid formation
  • Uniform single spheroid/EB formation in each well
  • Spheroid assay formation and analysis in the same plate
  • A variety of well bottom shapes: U-bottom, Spindle-bottom and V-bottom in 96 well format
  • High optical clarity plates for imaging
  • Stable, non-cytotoxic and cell non-adhesion surface
  • Easy handling, compatible with liquid robotic system
  • 384 well formats for high throughput assay
  • Compatible with bright-field and fluorescence imaging systems
  • White plates compatible with luminescent assays
PrimeSurface Key Benefits
Features

PrimeSurface series are coated with a unique ultra-hydrophilic polymer that covalently bound to plastic surface, and effectively inhibits cell attachment without cytotoxic and material degradation. The superior coating technologies and manufacturing processes offer uniform spheroid/EB formation and smooth surface to obtain clear cell images.


PrimeSurface Key Benefits

Three well bottom shapes of PrimeSurface 96 well plate


Three well bottom shapes of PrimeSurface 96 well plate

MS-9096

Retinal tissue formation from Human ES Cells using PrimeSurface 96 well V-bottom plate


Retinal tissue formation from Human ES Cells

Specifications
Cat. No. Microplates Product Name Number of wells Color Well bottom Maximum volume in each well Package (radiation sterilized)
MS-9096UZ PrimeSurface 96U 96 Clear U 300 μL Individually packed, 20 plates/case
MS-9096WZ PrimeSurface 96W 96 White U 300 μL Individually packed, 20 plates/case
MS-9096MZ PrimeSurface 96M 96 Clear Spindle 200 μL Individually packed, 20 plates/case
MS-9096VZ PrimeSurface 96V 96 Clear V 300 μL Individually packed, 20 plates/case
MS-9384UZ PrimeSurface 384U 384 Clear U 106 μL Individually packed, 20 plates/case
MS-9384WZ PrimeSurface 384W 384 White U 106 μL Individually packed, 20 plates/case


96 Slit-Well Plate

PHC is offering a new slit-well, ultra-low attachment 3D plate to facilitate easy handling of media exchange without disrupting spheroid formation.

Cell culturing involves frequent media replacement to provide nutrition to growing cells. In a standard 96 well ultra low cell attachment plate, media aspiration or dispensing has to be done extremely carefully to avoid disturbing the unattached spheroid, making this a time consuming operation.

With the introduction of PrimeSurface 96 Slit-Well Plate, media exchange for 96 well plates can be effciently handled with one step dispensing or aspiration for all 96 wells. This product can decrease pipetting time by over 80% while minimizing the risk of spheroid damage.


Key benefits
  • Generate and maintain uniform spheroids
  • Exchange media without disturbing spheroid formation
  • Minimize media exchange time by simultaneous delivery of cell culture media to all 96 wells
  • Use up to 1.5 times more media than in conventional plates, less media changes and more nutrients for the culture
PrimeSurface 96 Slit-Well Plate

Time Saving Design

Slit-Well structure allows simultaneous delivery of cell culture medium to all 96 wells.


Time Saving Design

Customer Testimony, Stanford University

“I found that the organoids grew equally sized in the slit well plate than with either 10cm dishes or traditional 96 well plates. The interconnectedness of the wells also ensures more biological consistency of the different wells. The benefit is the much faster time to feed cultures, which is a significant improvement over individual wells. Feeding also only requires a pipette, while feeding traditional wells requires using media boats and multichannel pipettes, which is much more waste and cost in supplies. This can add up for long term cultures. On the whole, I found them easy to use and very time, energy, and resource efficient.”



Features

Minimize media exchange effort and time without disturbing spheroid formation


Minimize media exchange effort

Generate and maintain uniform spheroids in long-term cultures


Minimize media exchange effort

Grow larger spheroids in the same well for long-term cultures.
Growing larger spheroids needs more media. Slit-Well plates allow 1.5 times more media volume compared to conventional plates providing more nutrients for larger spheroids.


Maximum capacity plates

Specifications
Cat. No. Microplates Product Name Number of wells Color Well bottom Maximum Well volume Package
MS-9096SZ PrimeSurface 96 Slit-Well Plate 96 Clear Spindle 0.3 ml Individually packed, 20 plates/case